Fig. 8
Evaluation of MS-275 treatment in a mouse model of EOC peritoneal metastasis. A In vivo experiment design. B Representative images of in vivo monitoring of SKOV3-luc-D3 cells in vehicle (n = 8) and MS-275 (n = 7) treated groups. Quantification of bioluminescence showed a significant tumor growth inhibition (TGI) in mice receiving MS-275 compared to the control group. C Representative images of parietal peritoneal tissues showing decreased tumor-emitting bioluminescence in MS-275 treated mice as compared to the vehicle group. Quantification of bioluminescence in parietal and visceral peritoneal tissues. The graph represents the mean average radiance (expressed as photons/s/cm2/sr) of SKOV3-luc-D3 cells ± SEM (*p < 0.05). D Parietal peritoneum samples were analyzed 5 weeks after i.p. injection of SKOV-luc-D3 cells. Haematoxylin & Eosin (H&E) staining shows a sub-mesothelial compact zone with accumulation of capillaries (arrows) in a vehicle mouse. A mainly conserved histological structure, without evidence of fibrosis and with a preserved MC monolayer was observed in MS-275 treated mice. Representative images of peritoneal serial sections of a mouse from the vehicle group show cytokeratin (CK) and α-SMA staining overlapping in the sub-mesothelial compact zone. Immunohistochemical analysis shows CK expression limited to the preserved mesothelium of a mouse treated with MS-275. Scale bar: 50 μm. CAFs: Carcinoma-associated fibroblasts. MCs: Mesothelial cells. E Representative images of parietal peritoneal tissues show decreased sub-mesothelial FN-1 staining in an MS-275 treated mouse (right) as compared to a control (left). Scale bar: 50 𝜇m. Right panel shows the quantification of FN-1 staining (right) (*p < 0.05)