Fig. 2

Analysis of Ru1 toxicity, PK and distribution in vivo. A-B Average weight ± SEM of mice treated orally (A) or retro-orbitally (r.o.) (B) with diluent control (Ctl) or Ru1 (1.4mg/kg,) for approximately 28-29 days. (*p < 0.05, as determined by unpaired two-sided Student’s t-test). C Average weight ± SEM of indicated organs extracted on d29 from mice treated with diluent control (Ctl) or Ru1 (1.4mg/kg, r.o). No significant differences were found, as determined by unpaired two-sided Student’s t-test. D Average values ± SEM of indicated hematocrit parameters determined from blood of mice extracted on d29 post treatment with diluent control (Ctl) or Ru1 (1.4mg/kg, r.o). No significant differences were found, as determined by unpaired two-sided Student’s t-test. E Picomoles of Ru1 per ml of serum, determined by ICP-MS, from mice at indicated time points post treatment initiation. F Picomoles of Ru1 per mg of tissue, determined by analyzing ruthenium with ICP-MS, from liver, kidneys and brain, extracted at indicated time points post treatment initiation. Dashed line indicates the background of the assay. G-H Indirect calorimetry analyses of mice treated with Ru1. Respiratory exchange ratio (RER) was determined as VCO2/VO2 and Energy expenditure (EE) was calculated as (3.185+ 1.232 x RER) x VO2. Shown are the mean RER (G) and mean EE (H) values ± SD for mice implanted with Azlet® Micro-Osmotic Pumps containing 5mM Ru1 or physiological saline (i.e., Sham) as a function of time (24 hours)