Fig. 5

CircSTX6 promotes BCa metastasis via the miR-515-3p/SUZ12 axis. A GSEA plot showed that the levels of SUZ12 expression were positively correlated with the metastasis ability. B-C The knockdown and overexpression efficiencies of SUZ12 were verified by qRT-PCR and western blot assays. D Representative and quantified results of the Transwell migration and invasion assays in the mock, SUZ12, scramble or sh-SUZ12#1 group. Scale bar, 100 μm. E-F Representative and quantified results of the Transwell migration and invasion assays in EJ cells transfected with vector or circSTX6, and those co-transfected with scramble or sh-SUZ12#1 plasmid. Scale bar, 100 μm. G Wound healing assays showed the migration abilities in EJ cells transfected with vector or circSTX6, and those co-transfected with scramble or sh-SUZ12#1 plasmid. Scale bar, 400 μm. H Representative and quantified results of the Transwell migration and invasion assays in UMUC3 cells transfected with vector or circSTX6, and those co-transfected with scramble or sh-SUZ12#1 plasmid. Scale bar, 100 μm. I Wound healing assays showed the migration abilities in UMUC3 cells transfected with vector or circSTX6, and those co-transfected with scramble or sh-SUZ12#1 plasmid. Scale bar, 400 μm. The data are presented as the means ± S.D. of at least three independent experiments. *P < 0.05. P values are calculated by Student’s t test in B, D and one-way ANOVA in B, F-I