Fig. 8

CircSTX6 improves the chemosensitivity of BCa to CDDP. A GSEA plot showed that the levels of SUZ12 expression were negatively correlated with the CDDP chemosensitivity. B Determination of IC50 values for CDDP treatment 24 h in EJ cells which were stably transfected with scramble or sh-SUZ12#1. C Flow cytometry apoptosis assays were used to assess apoptosis rate of EJ cells stably transfected with sh-SUZ12#1 upon CDDP treatment. D Determination of IC50 values for CDDP treatment 24 h in EJ cells which were stably transfected with scramble or sh-circ#1. E Flow cytometry apoptosis assays were used to assess apoptosis rate of EJ cells stably transfected with sh-circSTX6 after CDDP treatment. F-G Flow cytometry assay revealed the CDDP effect in EJ cells stably transfected with vector or circSTX6, and those co-transfected with scramble or sh-SUZ12#1. H Immunofluorescence staining showed the expression of γH2AX under CDDP treatment conditions in the indicated cells. The scale bar indicates 20 μm. I Representative images of xenograft tumors of circSTX6-knockdown group and their control group after being treated with either CDDP or PBS. J-K The tumor growth curves were drawn and tumor weight was measured. L-M Representative images of bioluminescence and histogram analysis of bioluminescence intensity in the indicated cell groups (n = 5 per group). N H&E staining was performed for histological confirmation of metastasizing tumor in lung. Scale bars, 200 μm. The data are presented as the means ± S.D. of at least three independent experiments. *P < 0.05. P values are calculated by Student’s t test in C and E and one-way ANOVA in G, H, K, M and N