Fig. 11

Effect of metformin and CR plus chemotherapy in subcutaneous KL PDX tumor: A, B Tumor growth inhibition in KL PDX implanted subcutaneously in immunocompromised mice. For three weeks metformin (MET) was administered daily at 300 mg/kg, cisplatin (DDP) 5 mg/kg once a week and caloric restriction (CR), consisted of 24 h of fasting, once a week. Caloric restriction alone or plus metformin and chemotherapy reduced (C) body weight and (D) blood glucose in immunecompromised mice. Two-way ANOVA was used for statistical analysis (**p < 0.01, *** p < 0.001). Error bars indicate SEM. The most statistically significant results have been reported in panel A. Single treatments, reported in panels B, did not induce a statistically significant reduction in tumor growth when compared to control or DDP groups