Fig. 2

Flow cytometry gating strategy for the identification of immune cell subsets in spleens and tumors: Total cells were first gated on physical parameters, a forward scatter (FSC)/side scatter (SSC) plot, and then gated cells are selected as singlets on the basis of SSC-A and SSC-H. Dead cells are excluded by the Zombie Aqua™ Fixable Viability Kit and leucocytes are identified as CD45⁺. Subset identification is shown for T cells (CD3⁺), CD8⁺ T cells, CD4⁺ T cells, myeloid cells (CD11b⁺), neutrophils (Ly6G⁺), and monocytes (Ly6C.⁺)