Fig. 3

In vitro effect of metformin and CR treatments on cell viability and metabolism: Cell viability measured by MTS assay after 72 h of treatment with 2 mM of metformin (MET) and 50% of caloric restriction (CR) in KRAS^G12D (A) and KRAS^G12D/LKB1^del (B) cell lines. One-way ANOVA was used for statistical analysis (**p < 0.01, *** p < 0.001). Error bars indicate SD. Seahorse XF Real-Time Assay was done after 24 h of treatment with 2 mM of metformin (MET) and 50% caloric restriction (CR) in KRAS^G12D (C) and KRAS^G12D/LKB1^del (D) cell lines. Two-way ANOVA was used for statistical analysis (* p < 0.05, **p < 0.01, *** p < 0.001). Error bars indicate SD