Fig. 5

In vitro effect of metformin and CR treatments on mTOR proliferative pathway: Representative immunoblot analysis of total and phosphorylated forms of P70 S6 kinase (P70) in KRAS^G12D (A) and KRAS^G12D/LKB1^del(B) cell lines. C, D Bar graphs of the quantification analysis of the active forms of P70 normalized on RAN, used as housekeeping. Proteins were extracted after 72 h of treatment with 2 mM of metformin (MET) and 50% caloric restriction (CR). Non-treated cells were used as control. One-way ANOVA was used for statistical analysis and no statistically significant results were highlighted. Error bars indicate SD