Fig. 7

DCAF13 knockdown inhibits the LUAD cell growth in vivo. (A) A549 shDCAF13 or shControl cells were implanted into BALB/C nude mice by subcutaneous injection. Photograph was shown as the xenograft tumor with shControl or shDCAF13 A549 cells. Tumors were separated after 5 weeks. (B) Mean tumor weights in mice. (C) Xenograft tumor growth curves in mice. (D) The mRNA expression of DCAF13, TP53, CDKN1A, and BAX in mouse tissues were detected by qPCR. (E) The protein expression of DCAF13 and p53 in mouse tissues were detected by western blot analysis. Data were statistically analyzed using Student’s t-test and values are shown as mean ± SD. **** p < 0.0001. (F) The protein expression of DCAF13, p53, p21, BAX, FAS, BBC3 and Ki67 in mouse tumor tissues were detected by immunohistochemistry assay. Representative images are shown, scale bar = 50 μm