Fig. 2

Endocytosis of NBTXR3 nanoparticles by cancer cells increases constantly over time. Following addition of 400 µM NBTXR3, the evolution of the cellular granularity at the indicated time-points was carried out by flow cytometry for the CT26.WT, HT1080, 42-MG-BA, and HCT116 cells. A Representative flow cytometry analysis of the evolution of the cellular granularity over time. B Kinetic of cellular granularity profile evolution for the CT26.WT (n = 4), HT1080 (n = 5), 42-MG-BA (n = 8), and HCT116 (n = 3) cells. Data of independent experiments are represented as the relative granularity to CTL ± SEM. Statistical test: One-way ANOVA. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001. C Baseline granularity of the tested cell lines. Data of independent experiments are represented as the mean granularity (SSC-A) ± SEM. D Comparison of granularity profile evolution for tested cell lines. Data of independent experiments are represented as the relative granularity to CTL ± SEM. E Evolution of the cellular granularity profile for 42-MG-BA cells (n = 4) according to NBTXR3 concentrations