Fig. 3

Endosomes containing NBTXR3 clusters are fused with lysosomes. Immuno-TEM images obtained 24 h after treatment of 42-MG-BA cells, showing that A NBTXR3 and B NBTXR3^RED clusters colocalize with LAMP-1, a specific marker for lysosomes. Black arrows indicate the location of AuNPs allowing identification of LAMP-1, surrounding the clusters of NBTXR3 and NBTXR3^RED. Scale bar, 300 nm. C Representative confocal microscopy images taken 24 h after treatment of CT26.WT, HT1080, 42-MG-BA and HCT116 cells with NBTXR3.^RED ., LysoTracker-stained lysosomes ;, NBTXR3 nanoparticle labelled with utilized Dextran Tetramethylrhodamine (NBTXR3^RED); , actin filaments; , nucleus. Scale bar, 10µm