Fig. 4

Endocytosis of NBTXR3 nanoparticles by cancer cells increases LysoTracker signal over the time. Following addition of 400 µM NBTXR3, the evolution LysoTracker signal at the indicated time-points was carried out by flow cytometry for the CT26.WT, HT1080, 42-MG-BA, and HCT116 cells. Representative evolution of LysoTracker signal over time analyzed by A flow cytometry and B fluorescent microscopy. C Kinetic of modification of LysoTracker profile for the CT26.WT (n = 4), HT1080 (n = 5), 42-MG-BA (n = 8), and HCT116 (n = 3) cells. Data of independent experiments are represented as the relative LysoTracker MFI to CTL ± SEM. Statistical test: One-way ANOVA or Friedman test. *, p < 0.05; **, p < 0.01; ***, p < 0.001, p < 0.0001; ****. D Baseline LysoTracker signal of the tested cell lines. Data of independent experiments are represented as the MFI ± SEM. E Comparison of MFI LysoTracker profile evolution for tested cell lines. Data of independent experiments are represented as the relative MFI to CTL ± SEM. F Evolution of the MFI LysoTracker profile for 42-MG-BA cells (n = 4) according to NBTXR3 concentrations