Fig. 5

Endocytosis of NBTXR3 nanoparticles induces upregulation of genes linked to lysosomes. RNAseq analysis of CT26.WT cells treated for 24 h with NBTXR3 (n = 3). A Heatmap of lysosome-associated genes (GO:0005764). B Volcano plot of lysosome-associated genes (GO:0005764) showing significantly differentially expressed genes (p < 0.05) of NBTXR3-treated (NBTXR3) vs. untreated (CTL) CT26.WT cells. Heatmap of expression of C primary lysosome (GO:0005766) D secondary lysosome (GO:0005767) and E late endosome to lysosome transport (GO:1,902,774). For all presented heatmaps, each column stands for the mean value of three independent experiments. Expression values are shown as the Z-score mean of the log₂ transformed normalized counts. Red and green are assigned to higher and lower expression, respectively, according to the color key in the right