Fig. 6

Intratumoral injection of NBTXR3 lead to significant increase of both granularity and LysoTracker signal, and endocytosis by cancer cells. A Schematic representation of the experiment workflow. Box and whisker plots representation of cancer cells B granularity and C LysoTracker signal measured 24 h after intratumoral injection of NBTXR3 or 5% Glc (vehicle, CTL). Presented data were obtained from 4–5 mice per group (each dot represents one value) from two independent experiments. Statistical test: Unpaired t-test. *, p < 0.05. D TEM representative images of NBTXR3 nanoparticles uptake by CT26.WT cells 24 h after intratumoral injection of NBTXR3 from five mice (CTL, mouse #1; NBTXR3-treated mice: mouse #2, pictures 1–2; mouse #3, picture 3; mouse #4, picture 4; mouse #5, pictures 6–8). White arrow: membrane ruffles enclosing a small NBTXR3 nanoparticle aggregate; black arrow: internalized NBTXR3 nanoparticles cluster. Scale bars: CTL, 2µm; 1–8, 0.5µm. Abbreviations: CTL, control cells (no NBTXR3); Cyt, cytoplasm; Nuc, nucleus