Fig. 8

NBTXR3 + RT promotes ferroptosis cell death through lysosomal membrane permeabilization and lipid peroxidation enhancement. A Clonogenic assay on CT26.WT (n = 5), HT1080 (n = 4), 42-MG-BA (n = 3) and HCT116 (n = 5) cells. Data of independent experiments are represented as the surviving fraction ± SEM. Statistical test: One way ANOVA. B Relative LysoTracker MFI signal measured by flow cytometry 1 h post-RT in CT26.WT (n = 4), HT1080 (n = 3), 42-MG-BA (n = 3) and HCT116 (n = 4) cells to evaluate lysosomal membrane permeabilization. Data of independent experiments are represented as the MFI ± SEM. Statistical test: One way ANOVA. C Fluorescent microscopy representative images of Cathepsin D signal in 42-MG-BA cells 6h post-RT, at the indicated condition. Scale bar, 10µm. D Scatter dot plot of Cathepsin D signal intensity measured in 42-MG-BA cells at the indicated condition, from three independents experiments. Each dot represents one cell intensity. “n” on figures indicates number of cells analyzed. Statistical test: Kruskal–Wallis test. Bar, median. E Relative BODIPY MFI signal measured by flow cytometry 24 h post-RT in CT26.WT, HT1080, 42-MG-BA and HCT116 cells measured, to evaluate lipid peroxidation. Data of independent experiments (n = 3) are represented as MFI ± SEM. Statistical test: One way ANOVA. For all Figures: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, non-significant