Fig. 3

Bioinformatics analysis of the expression levels of miR-204-5p and BRD4. A The binding sites of AL139294.1 and miR-204-5p, as well as miR-204-5p and BRD4. TCGA cohort (TCGA-LUADLUSC dataset) shows lower levels of miR-204-5p in lung cancer tissues than those in normal tissues (B, normal, n = 91; lung cancer, n = 999) as well as paired tissues (C, normal, n = 91; lung cancer, n = 91). D ROC analyses were used to evaluate the diagnostic ability of miR-204-5p to distinguish normal (n = 46) and LUAD (n = 521) tissues in the TCGA cohort (TCGA-LUAD) dataset. Analyses of the TCGA cohort (TCGA-LUADLUSC dataset) show BRD4 mRNA levels in normal tissues (n = 108) and lung cancer tissues (E, n = 1037) as well as paired tissues (F, normal, n = 106; lung cancer, n = 106). G ROC analyses were used to estimate the diagnostic efficacy of BRD4 in distinguishing normal (n = 347) and LUAD (n = 515) tissues in the TCGA cohort and GTEx cohort (TCGA_GTEx-LUAD dataset). H Survival analyses (Kaplan–Meier plotter (kmplot.com)) show the association of BRD4 mRNA levels with overall survival (n = 1411) of patients with lung cancer. The cutoff is an auto-cutoff at which a significant difference is obtained between the lower and higher expression levels. The data were transformed by Log 2. **P < 0.01