Fig. 4

Role of miR-204-5p in the development of NSCLC. A The transfection efficiency of miR-204-5p mimics and inhibitors in Beas-2B and NCI-H1299 cells was checked by qPCR. B The proliferation ability of cells transfected with miR-204-5p mimics or inhibitors was measured by CCK-8 assay. Wound healing assay (C) and transwell assay (D) were used to measure the migration and invasion of NSCLC cells transfected with miR-204-5p mimics or inhibitors. E EMT-related markers were detected by western blotting after the knockdown or overexpression of miR-204-5p. F Colony formation assay was used to detect the clone formation ability of Beas-2B and NCI-H1299 cells after miR-204-5p knockdown or overexpression. G Schematic diagram shows the predicted binding sites of miR-204-5p with the 3’UTR of wild-type and mutant BRD4. H Luciferase reporter assay was conducted to test the binding of miR-204-5p with the 3’UTR of wild-type or mutant BRD4 in HEK-293 T cells. qPCR (I) and western blotting (J) were performed to detect BRD4 mRNA and protein levels after the transfection of miR-204-5p mimics or inhibitors, respectively. *P < 0.05, **P < 0.01