Fig. 6

AL139294.1 promotes the tumorigenic capacities of the NSCLC cells through the miR-204-5p–BRD4–Wnt5a and miR-204-5p–BRD4–NF-κB2 pathways. A The levels of AL139294.1 in Beas-2B and NCI-H1299 cells were quantified after the transfection of pcDNA3.1 empty vectors or AL139294.1-overexpression plasmids. B The effects of AL139294.1 overexpression, miR-204-5p mimics, and their co-transfection on the proliferation ability of Beas-2B and NCI-H1299 cells were examined by CCK-8 assay. Wound healing assay (C) and transwell assay (D) were used to evaluate the migration and invasion of Beas-2B and NCI-H1299 cells transfected with AL139294.1-overexpression plasmids and miR-204-5p mimics. E Western blotting was carried out to detect BRD4 and EMT-related proteins after the transfection with AL139294.1-overexpression plasmids and miR-204-5p mimics. F Colony formation assay was performed to evaluate the colony formation ability of Beas-2B and NCI-H1299 cells. G The mRNA levels of BRD4 in cells were detected by qPCR after the transfection of AL139294.1-overexpression plasmids and miR-204-5p mimics. H Western blotting was used to test the levels of Wnt5a pathway-related proteins (Wnt5a, β-catenin, AKT, and JNK) and NF-κB2 pathway-related proteins (NF-κB2, and SPP1) in Beas-2B and NCI-H1299 cells treated with AL139294.1-overexpression plasmids, miR-204-5p mimics or JQ1. *P < 0.05, **P < 0.01