Fig. 7

The transport of AL139294.1 by EVs and the effect of AL139294.1 in EVs on NSCLC. A Dil-labeled EVs (secreted by NCI-H1299 and LTEP-A2 cells, in red) and Beas-2B cells (transfected with Rab5 plasmids, in green) were co-cultured. The co-localization of EVs and Rab5 was photographed. B NCI-H1299 and LTEP-A2 cells were transfected with AL139294.1-overexpression plasmids with a GFP-tagged (in green), and the secreted EVs (in red) were co-cultured with Beas-2B cells. The co-localization of AL139294.1 and EVs in Beas-2B cells was imaged. C The levels of AL139294.1 in Beas-2B, NCI-H1299, LTEP-A2 cells, and their secreted EVs were quantified by qPCR. D The levels of AL139294.1 in secreted EVs from cells transfected with AL139294.1 si202 or overexpression plasmids were quantified by qPCR. E A co-culture system was used to study the effect of EVs secreted by treated NCI-H1299 or LTEP-A2 cells on Beas-2B cells. F, G CCK-8 assay was performed to evaluate Beas-2B cells’ proliferation ability. Wound healing assay (H) and transwell assay (I) were used to evaluate the migration and invasion ability of Beas-2B cells. J Colony formation assay was used to detect the clone formation ability of Beas-2B cells. K Western blotting was conducted to check the BRD4 and EMT-related proteins in Beas-2B cells after the co-culture. *P < 0.05, **P < 0.01