Fig. 7

APOL1 partly reverses tumor progression and lipid storage initiated by HIF2α/ LINC02609 axis in vitro and in vivo. (A) WB analysis of HIF2α and APOL1 in renal cancer cell 786-O and A498 with indicated cells. (B) MTS assays revealed cell growth curves of indicated cells. (C) Photomicrographs of Oil Red O-stained in indicated cells (Magnification: 200× & 400×) and quantification of ORO in indicated cell lines. (D) Migration and invasion assays for indicated renal cancer cells. Number of migrated cells was quantified in three random images from each treatment group. (E, F) 786-O cells expressing indicated plasmid were transplanted into mice. Tumor weight of each nude mouse at the end of 49 days. (G) Representative images of livers of nude mice at the 30th days after IV. injection of indicated renal cancer cell. Quantification analysis of number of metastatic nodules. (H) Schematic model of HIF-2α/Apol1 mediated lipid storage promotes endoplasmic reticulum homeostasis and regulates tumor progression in ccRCC