Fig. 3
From: EMID2 is a novel biotherapeutic for aggressive cancers identified by in vivo screening
EMID2 incorporation in the extracellular matrix modifies its mechanical properties with consequent cytoskeletal re-organization. A. Rheological analysis of Matrigel polymerization in presence of recombinant EMID2 (rEMID2, red) compared to control Matrigel (black). G’, elastic modulus; G’’, viscous modulus. B. Strain sweep of Matrigel in the presence of rEMID2 (red) or control (black). Arrows indicate breaking points. C. Decellularized ECM secreted by primary fibroblasts cultured in a medium containing rEMID2 or in a control medium. D. Representative images of LG cancer cells seeded on Matrigel either in the absence or in the presence of rEMID2. E. Quantification of the number of stress fibers per cell, identified by F-actin, in cancer cells seeded on Matrigel either in the absence or in the presence of rEMID2. F. Quantification of the length of focal adhesions, identified by paxillin, in cancer cells seeded on Matrigel either in the absence or in the presence of rEMID2. G. NIH-3T3 fibroblasts cultured on the decellularized ECM. H, I. Quantification of the number and length of filopodia protruding from NIH-3T3 fibroblasts. J. Representative images of LG cancer cells seeded on Matrigel either in the absence or in the presence of rEMID2. K. Quantification of the ratio of YAP integrated density in the nucleus and cytoplasm of LG cells as in J. Scale bar in C, G, J 50 μm, in D 100 μm. In E, F, H, I, K, data are shown as mean ± SEM and were analyzed by Student t-test. *p < 0.05, **p < 0.01