Fig. 4

Effects of MP1 on translation pathway. A Western blot images for the expression of translation/mTOR components signaling in two MYC-amplified MB cell lines following treatment with MP1 in a dose-dependent manner for 24 h. Cyclophillin B was used as the loading control in these experiments. Bar graphs show the quantification of expression of key target proteins (shown in western blot images) relative to the DMSO (“0” treatment) control in the combined blots of HD-MB03 and D-341 cells after Cyclophillin B (loading control) normalization using Image-J software. The values represent the mean ± SEM of three blot replicates. The values represent the mean ± SEM of three blot replicates. *p < 0.05; **p < 0.01; ***p < 0.001 (Student t test, DMSO vs. MP1 treatments). B Overall protein synthesis measurement by OPP-incorporation following treatment with MP1 for 24 h. CHX (50 μg/ml, 1 h) was used as a positive control for protein synthesis inhibition. Values represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.005 (Student-t-test)