Fig. 5

MP1 treatment alters energy metabolism in MB. A Heatmap showing top 60 metabolites altered in HD-MB03 cells treated with MP1 (0.25 µM) in triplicate for 24 h. Color intensity represents the magnitude of alteration in individual metabolites. Scale for color intensity is shown adjacent to the heatmap. B Pathway analysis showing significantly altered metabolic pathways in MP1 treated HD-MB03 cells, compared to DMSO solvent. Scale under the pathway plot shows the fold enrichment and color scale adjacent to pathway plot indicates significance (p-value) for altered pathways. C Extracellular acidification rate (ECAR) analysis for glycolytic activities in HD-MB03 cells after treatment with 0.25 µM MP1 for 24 h. G, glucose; O, oligomycin; 2-DG, 2-deoxyglucose. The bar graphs show glycolytic capacity and glycolytic reserve activities derived from ECAR activities shown in line graph. The results represent the mean ± SEM of three replicates. **p < 0.01 (Student t test, DMSO vs MP1). D Oxygen consumption rate (OCR) analysis for mitochondrial oxidative phosphorylation status in HD-MB03 cells after treatment with 0.25 µM MP1 for 24 h. O, oligomycin; F, FCCP; A/R, antimycin/rotenone. The bar graphs show maximal respiration and ATP production activities derived from OCR activities shown in line graph. The results represent the mean ± SEM of three replicates. **p < 0.01 (Student t test, DMSO vs MP1)