Fig. 1

NEURL3 is hypermethylated in NPC. (a) Heatmap clustering of the top 20 hypermethylated genes based on integrated analysis of two genome-wide DNA methylation microarray datasets (GSE52068 and GSE62336) in NPC and normal nasopharyngeal tissues. (b) The genome features of NEURL3 observed using the UCSC genome browser and schematic diagram of the CpG island sites at NEURL3 promoter region. (c) Methylation levels of NEURL3 in NPC tissues (n = 24) and normal tissues (n = 24) in the methylation microarray dataset GSE52068. (d) Methylation levels of NEURL3 in NPC tissues (n = 25) and normal tissues (n = 25) in HongKong methylation microarray dataset GSE62336. (e) Representative images of bisulfite pyrosequencing analysis of the NEURL3 promoter region. (f) Statistical analysis of methylation levels of NEURL3 in NPC (n = 8) and normal (n = 8) tissues. Data in c, d, and f are presented as the mean ± SD, and the p-values were determined by Student’s t-test (*p < 0.05)