Fig. 3

NEURL3 suppresses NPC cell migration, invasion, and EMT in vitro. (a) In GSEA analysis using a public RNA-seq dataset (GSE102349), the gene sets associated with cell migration and metastasis pathways were significantly enriched in NPC samples with a low NEURL3 expression. (b) The migratory abilities of HONE1, SUNE1, and HK1 cells transfected with HA-NEURL3 plasmid or its empty vector determined by wound healing assay. (c-d) The migratory (c) and invasive (d) abilities of HONE1, SUNE1, and HK1 cells transfected with HA-NEURL3 plasmid or its empty vector determined by Transwell assay. (e) IF staining showing the filopodia length on HONE1, SUNE1, and HK1 cells transfected with HA-NEURL3 plasmid or its empty vector. (f) The morphology changes induced by TGF-β1 in HONE1, and SUNE1 cells transfected with HA-NEURL3 plasmid or its empty vector. Scale bar, 50 μm. Data of b, c, d, and e are shown as mean ± SD, and the p-values were determined by Student’s t-test (*p < 0.05)