Fig. 6

Restoration of Vimentin reverses the tumor suppressive effect of NRURL3. HONE1, SUNE1, and HK1 cells were transiently co-transfected with HA-NEURL3 or empty vector together with Flag-Vimentin or empty vector. (a) The migratory abilities of transfected NPC cells were determined by wound healing assay. (b-c) The migratory (b) and invasive (c) abilities of transfected NPC cells were determined by Transwell assay. (d) IF staining showing the pseudopods on transfected NPC cells. (e) The morphology changes induced by TGF-β1 in transfected NPC cells. Scale bar, 50 μm. Data of a, b, and c are shown as mean ± SD, and the p-values were determined by Student’s t-test (*p < 0.05)