Fig. 2

MAGOH was generally upregulated in GC and predicted poor prognosis. A Relative expression of MAGOH in GC tissues identified by label-free relative quantitative proteomics. B Relative expression of MAGOHB in GC tissues identified by label-free relative quantitative proteomics. C Differences in the expression of MAGOH in GC tissues analyzed using the GEPIA database. D Differences in the expression of MAGOHB in GC tissues analyzed using the GEPIA database. E The Kaplan‒Meier plotter database (210092_at) was used to analyze the correlation between the MAGOH level and overall survival (OS) or first progression survival (FPS) in GC patients. F The Kaplan‒Meier plotter database (218894_s_at) was used to analyze the correlation between MAGOHB levels and OS or FPS in GC patients. G qRT‒PCR analysis of MAOGH expression in GC tissues and corresponding normal tissues (n = 60, p < 0.0001; Student’s t test). H Correlation between MAGOH expression and tumor stage (I-II or III-IV) in 60 GC tumor samples. The statistical significance of the data was analyzed by the chi-square test. I Correlation between MAGOH expression and N stage (N0-1 or N2-3) in 60 GC tumor samples. The statistical significance of the data was analyzed by the chi-square test. J Western blotting was used to analyze the expression level of the MAGOH protein in GC tissues and adjacent normal tissues (n = 18). K ImageJ quantification of the WB results for the MAGOH protein (n = 18). The data are shown as the means ± SDs. Differences were considered significant if p < 0.05 (**p < 0.01, ***p < 0.001, ****p < 0.0001)