Fig. 3

MAGOH promoted GC proliferation and metastasis in vitro. A A CCK-8 assay showed that MAGOH knockdown inhibited the proliferation of GC cells. B The EdU immunofluorescence assay showed that MAGOH knockdown inhibited the proliferation of GC cells. Scale bar = 100 μm. C Flow cytometry showed that MAGOH knockdown accelerated the apoptosis of GC cells. The bar graph (right panel) showed the percentage of apoptotic cells. D Transwell assays revealed that MAGOH knockdown inhibited the migration and invasion abilities of GC cells. Scale bar = 200 μm. E A CCK8 assay showed that MAGOH overexpression promoted the proliferation of GC cells. F The results of the EdU immunofluorescence assay showed that MAGOH overexpression enhanced the proliferation of GC cells. Scale bar = 100 μm. G Flow cytometry showed that MAGOH overexpression decreased the apoptosis of GC cells. The bar graph (right panel) showed the percentage of apoptotic cells. H Transwell assays showed that MAGOH overexpression facilitated the migration and invasion of GC cells. Scale bar = 200 μm