Fig. 4

MAGOH encouraged GC tumor growth and distant metastasis. A Schematic representation of the subcutaneous xenograft tumor model in BALB/c nude mice. B The mRNA levels of MAGOH in xenograft tumors from nude mice were determined by RT‒qPCR (n = 5). C The protein levels of MAGOH in xenograft tumors from nude mice were determined via WB (n = 5). D, E Anatomical images of subcutaneous xenograft tumors in different groups. F, G Tumor growth curves and weight analyses of xenografts in nude mice. H Xenograft tumor sections were stained with HE and subjected to IHC using anti-MAGOH, anti-Ki67, and anti-Bcl-2 antibodies. Scale bar for 40X-magnified images = 500 μm; scale bar for 200X-magnified images = 100 μm. I Schematic diagram of the process used to establish a pulmonary metastasis model in BALB/c nude mice after tail vein injection. J Pulmonary metastasis models were constructed with MAGOH-knockdown (sh-MAGOH) or negative control (sh-NC) AGS cells (n = 5). Representative photographs of the dissected lungs (left) were presented to show metastases (marked by black arrows), and HE staining was performed to confirm the presence of metastases (middle); the results were presented in histograms (right). K Schematic diagram of BALB/c nude mice after spleen vein injection to establish a liver metastasis model. L Liver metastasis models were constructed with MAGOH-knockdown (sh-MAGOH) or negative control (sh-NC) AGS cells (n = 5). Representative photographs of the dissected livers (left) were presented to show metastases (marked by black arrows), and HE staining was used to confirm the presence of metastases (middle), which were quantified in histograms (right)