Fig. 5

MAGOH indirectly regulated the formation of RONΔ160. A The correlation between MAGOH and RONΔ160 expression in GC tissues and paired normal tissues was analyzed. B RT‒qPCR was used to measure the mRNA levels of RON∆160 and flRON in GC cells transfected with MAGOH siRNA. C RT‒qPCR was used to measure the mRNA levels of RON∆160 and flRON in GC cells transfected with the MAGOH overexpression plasmid. D WB was used to measure the protein levels of RON∆160 and flRON in GC cells transfected with MAGOH siRNA, the quantified results were presented in histograms (right). E WB was used to measure the protein levels of RON∆160 and flRON in GC cells transfected with a MAGOH overexpression plasmid, the quantified results were presented in histograms (right). F Biotinylated RON pull-down assays of AGS and Kato III cell lysates were performed, and the expression levels of EJC components, including MAGOH, EIF4A3, and Y14, were measured via WB. G RIP analysis of RON was performed using IgG and MAGOH antibodies. The relative enrichment of RON mRNA was calculated by qRT‒PCR