Fig. 7

Silencing hnRNPA1 rescued the changes in cell proliferation and invasion caused by MAGOH knockdown. A, B AGS cells (A) and Kato III cells (B) were transfected with NC siRNA or MAGOH siRNA, and RIP analyses of RON in both groups were performed using anti-IgG and anti-hnRNPA1 antibodies, respectively. The relative enrichment of RON mRNA was calculated by qRT‒PCR. C, D qRT‒PCR (C) and WB (D) were used to assess the expression of RON∆160 and flRON in the MAGOH-silenced and hnRNPA1-silenced rescue groups of AGS cells. E, F qRT‒PCR (E) and WB (F) were used to assess the expression of RON∆160 and flRON in the MAGOH-silenced and hnRNPA1-silenced rescue groups of Kato III cells. G, H A CCK8 assay was conducted to analyze the short-term proliferation ability of AGS cells (G) and Kato III cells (H) after cotransfection with si-NC + si-NC, si-NC + si-hnRNPA1, si-NC + si-MAGOH or si-hnRNPA1 + si-MAGOH. I, J A colony formation assay was conducted to evaluate the long-term proliferation ability of cotransfected AGS cells (I) and Kato III cells (J). K, L A Transwell assay was performed to evaluate the invasion and migration capacities of cotransfected AGS cells (K) and Kato III cells (L). Scale bar = 200 μm