Fig. 1

LINC00571 is upregulated in TNBC tissues and breast cancer cell lines. A Heatmap representing the diverse expression patterns of long non-coding RNAs across five pairs of triple-negative breast cancer tissues. B Volcano plot representing the diverse expression patterns of 13 long non-coding RNAs across five pairs of triple-negative breast cancer tissues. C Venn diagram illustrating the intersection between RNA-seq data derived from 5 pairs of triple-negative breast cancer tissues and prognostic data extracted from the TCGA database for triple-negative breast cancer. D PCR analysis revealing the relative abundance of lncRNAs in human breast cancer epithelial cell lines (MCF10A) and triple-negative breast cancer (TNBC) cell lines (MDA-MB-231, BT-549). GAPDH was utilized as an internal control. E Expression level assessment of LINC00571 in triple-negative breast cancer (TNBC) compared to adjacent normal breast tissues, performed using qRT-PCR analysis. F Determination of LINC00571 nuclear and cytoplasmic distribution by qRT-PCR analysis in MDA-MB-231 and BT-549 cells, Cytoplasmic and nuclear controls were GAPDH and U6, respectively. G RNA-FISH assay revealing the cytoplasmic localization of LINC00571 within MDA-MB-231 and BT-549 cells. Positive controls for cytoplasm (18S) and nucleus (U6) were labeled with Cy3 (red), while the LINC00571 probe was labeled with FITC (green). Nuclei were counterstained with DAPI (blue). scale bar :10μm. Statistical analyses are depicted in bar graphs. Data are presented as mean ± SD from three independent experiments. Significance levels are denoted as * for p<0.05, ** for p<0.01, and *** for p<0.001, as determined by the t-test