Fig. 2

LINC00571 regulates proliferation and apoptosis of TNBC cells. A-C Cellular proliferation rates were assessed using CCK-8, colony formation, and EdU assays, revealing the impact of shNC or shLINC00571 and vector or LINC00571 in MDA-MB-231 cells, n=3. scale bar: 50μm. D Flow cytometry-based cell cycle analysis conducted on MDA-MB-231 cells stained with propidium iodide (PI), n=3. E Apoptosis evaluation carried out through a flow cytometry assay on MDA-MB-231 cells stained with Annexin V (FITC) and propidium iodide (PI), n=3. F Photographs of xenograft s captured with a digital camera. MDA-MB-231 cells were subcutaneously injected into BALB/c athymic nude mice, n=5. Volume was monitored every ten days and calculated as volume = length × (width)²/2. G Immunohistochemical images showing Ki67 and PCNA staining in shNC or shLINC00571 groups (left) and vector or LINC00571 groups (right), scale bar: 100μm. Quantification of positive Ki67 and PCNA, n = 5. H Immunofluorescence images illustrating TUNEL staining in shNC or shLINC00571 groups (left) and vector or LINC00571 groups (right), scale bar: 60μm. Quantification of positive TUNEL, n = 5. Statistical analyses are depicted in bar graphs. Data are presented as mean ± SD. Significance levels are denoted as * for p<0.05, ** for p<0.01, and *** for p<0.001, as determined by the t-test