Fig. 3

LINC00571 promotes the progression of TNBC by TCA signaling pathway. A Gene set enrichment analysis (GSEA) of the TCGA dataset using the TCGA dataset revealed the top 10 pathways associated with high expression of LINC00571. B GSEA plots showed a positive correlation between LINC00571 expression and the set of upregulated genes in tricarboxylic acid (TCA) cycle within triple-negative breast cancer. C PCR analysis revealed the expression profile of corresponding genes in MDA-MB-231 cells with LINC00571 knockdown (left) or LINC00571 overexpression (right), n = 3. D Left, oxygen consumption rate (OCR) was analyzed in MDA-MB-231 cells with LINC00571 knockdown (n = 4). Right, basal respiration, ATP-coupled respiration and maximal respiration (n= 4). E Left, oxygen consumption rate (OCR) was analyzed in MDA-MB-231 cells with LINC00571 overexpression (n = 4). F-G Relative lactate level (F) and relative ATP level (G) in MDA-MB-231 cells with LINC00571 knockdown (left) or LINC00571 overexpression (right) were shown, n = 3. (H) Relative lactate level (F) and relative ATP level (G) in MDA-MB-231 cells with LINC00571 knockdown (left) or LINC00571 overexpression (right) were shown, n = 3. Statistical analyses are depicted in bar graphs. Data are presented as mean ± SD. Significance levels are denoted as * for p<0.05, ** for p<0.01, and *** for p<0.001, as determined by the t-test