Fig. 8.

LINC00571 mediates IDH2 expression to regulate the progression of TNBC cells. A-C Proliferation status of MDA-MB-231 cells co-transfected with shLINC00571 and IDH2 was assessed using CCK-8, colony formation assays, and EdU assays, n = 3. scale bar: 50μm. (D) Cell cycle analysis was conducted via flow cytometry, and MDA-MB-231 cells were stained with propidium iodide (PI), n = 3. E Apoptosis was detected using a flow cytometry assay, with Annexin V and propidium iodide (PI) staining in MDA-MB-231 cells, n = 3. F-J In vivo studies involved subcutaneous injection of MDA-MB-231 cells co-transfected with shLINC00571 and IDH2 into BALB/c athymic nude mice, n =5. Images of xenograft tumors were captured using a digital camera (F). Tumor weight was measured on day 50 (G). Tumor volume was monitored every ten days, calculated using the formula: volume = length × (width)²/2 (H). Immunohistochemical images of Ki67 and PCNA staining were conducted for control, shLINC00571-, IDH2-, and shLINC00571 combined plus IDH2-treated groups. scale bar: 100μm (I). Immunofluorescence images illustrated TUNEL staining in control, shLINC00571-, IDH2-, and shLINC00571 combined plus IDH2-treated groups. scale bar: 60μm (J). Statistical analyses are depicted in bar graphs. Data are presented as mean ± SD from three independent experiments. Significance levels are denoted as * for p<0.05, ** for p<0.01, and *** for p<0.001, as determined by the t-test