Fig. 1
Significant differences in lipid metabolites are detected in murine and human AITL Tfh cells compared to healthy T cells. CD4 + PD-1high cells were isolated from pLck-GAPDH mouse lymphoma and compared to WT CD4 + splenocytes for metabolite analysis. Principle component analysis for the two groups in is shown in (A) and data are represented in the volcanoplot (B). C Heatmap of the 50 metabolites showing more than 1.5 fold log2 change between the mAITL CD4 + PD-1high T cells and splenic CD4 + T cells. D Lipid metabolism pathway analysis for the GSEA data from AITL patient (n = 8), healthy Tfh cells (n = 14), central memory T cells (Tcm) (n = 6), effector memory T cells (Tem) (n = 6), naive T cells (Tn) (n = 6), regulatory T cells (T reg) (n = 13) and stem cell memory T cell (Tscm) (n = 6). Bubble representation (Bubbles size and numbers represents the sample enrichment score (SES); p-values are indicated in supplementary Fig. 1A)