Fig. 3

Transcriptome sequencing revealed that KPNB1 knockdown reduced the expression of NLGN3 in glioblastoma. A. U251MG cells infected by siControl or siKPNB1 were harvested for transcriptomic RNA sequencing. Boxplots show the quality control of RNA sequencing. B-C. Volcano plot (B) and heat map (C) show that NLGN3 was significantly down-regulated in KPNB1-silenced U251MG cells. D. GO functional enrichment analysis was performed and KPNB1 was associated with multiple synaptic activities in U251MG cells. E. TCGA (n = 701) database correlation analysis showed that there was a positive correlation between the mRNA expression levels of NLGN3 and KPNB1