Fig. 8

USP7/KPNB1/YBX1/NLGN3 axis promoted tumorigenesis in GBM. A, D, and F. U87MG and U251MG cells were infected with shUSP7, shKPNB1, or both. Cells were collected for Western blot (A), colony formation assay (D), and CCK8 assay (F). Data expressed as mean ± SD, n = 10 per group. *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA. B. U87MG and U251MG cells were treated with P5091 and then collected for Western blot. C, E, and G. U87MG and U251MG cells were infected with Control or shKPNB1 for 48 h. Then, cells were transfected with pcDNA3.1 or Myc USP7 as indicated. After 24 h, cells were harvested for Western blotting analysis (C), colony formation assay (E), and CCK8 assay (G). Data expressed as mean ± SD, n = 10 per group. *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA. H. U251MG cells were infected with shUSP7 and shKPNB1. After puromycin selection, cells were administered to nude mice via intracranial injection to establish a xenograft model. Representative bioluminescence imaging of a tumor at day 28 is shown. I. Quantitative assessments of tumor growth following implantation. Data expressed as mean ± SD, n = 10 per group. *P < 0.05; **P < 0.01; ***P < 0.001, one-way ANOVA. J. Schematic diagram summarizing the role of the USP7/KPNB1/YBX1/NLGN3 axis in promoting tumorigenesis in GBM