Fig. 3

Expression of MHC-I, MICA/B, ULBP-2/5/6, and B7-H6 on surfaces of BCa and urothelial cells. (A) MHC-I expression on human BCa cell lines, including BIU-87, T24, UMUC-3, 5637, and normal urothelial cell line SV-HUC-1, were detected using flow cytometry. Representative images and summary data of the MFI for MHC-I are shown (n = 3); (B) MICA/B expression on human BCa cell lines and normal urothelial cell line SV-HUC-1 were detected using flow cytometry. Representative images and summary data of the percentages of MICA/B + cells are shown (n = 3); (C) ULBP-2/5/6 expressions on human BCa cell lines and normal urothelial cell line SV-HUC-1 were detected using flow cytometry. Representative images and summary data of the percentages of ULBP-2/5/6 + cells are shown (n = 3); (D) B7-H6 expression on human BCa cell lines and normal urothelial cell line SV-HUC-1 was detected using flow cytometry. Representative images and summary data of the percentages of B7-H6 + cells are shown (n = 3). All bars represent the means ± SD. Statistical significance was determined using an unpaired t-test (A-D). *p < 0.05. Abbreviations: MHC-I: major histocompatibility complex class I, MICA/B: MHC-I polypeptide-related sequences A and B, ULBP-2/5/6: cytomegalovirus UL16-binding protein-2/5/6; BCa: bladder cancer