Fig. 8

Receptors of the chemokines expressed on CD3⁺ T cells and T cell count migrating across the transwell after blocking CCL1/2/20 in the supernatant of coculture medium. (A) The expressions of the chemokine receptors on CD3⁺CD56⁻ T cells were detected using flow cytometry (n = 3 for all molecules). The representative images are shown. (B) Summary data of the cumulative numbers of T cells determined using a fluorescence cell analyzer after being harvested from the lower chambers, which were added into blocking antibodies against CCL1 (0.25 µg/mL), CCL2 (40 ng/mL), or CCL20 (10 µg/mL) (n = 3 for each). Data are shown as mean ± SD. Statistical significance was determined using an unpaired t-test. *p < 0.05. Abbreviations: CXCR: C-X-C chemokine receptor; CCR:C-C chemokine receptor; XCR:X-C chemokine receptor