Fig. 1

CDH17 associates with DSC1. A RKO and HT-29 cells were transfected with vectors encoding for CDH17. Transfectants were subjected to immunoprecipitation (IP) using anti-CDH17 or control antibodies, and the IPs were analyzed by Western blot to detect CDH17. B KM12SM and SW620 cells were lysed and the extracts subjected to IP as in A. IPs were analyzed by Western blot to confirm DSC1 and CDH17 co-immunoprecipitation. C Immunofluorescence showing the co-localization of CDH17 and DSC1 in KM12SM and SW620 cells. D Western blot analysis of the indicated CRC cell lines to detect DSC1 and CDH17. RhoGDI was used as a loading control. E The indicated cell lines were transfected with control or α2 integrin-targeting siRNAs. The α2 integrin expression levels were significantly inhibited by the siRNA transfection (***, p < 0.001). F Cell lysates from the transfectants were subjected to IP as in A. IPs were analyzed by Western blot to detect DSC1 and CDH17. Data are representative of three independent experiments