Fig. 3

CDH17 overexpression regulates cell adhesion, migration and invasion in colon cancer cells. A The indicated cancer cell lines were transfected with empty vectors (Mock) or vectors encoding for the mutant CDH17 RAD. Cell extracts were analyzed by Western blot to detect CDH17, DSC1 and, as loading control, RhoGDI. CDH17 expression levels were significantly increased by the transfection of CDH17 RAD vectors (*, p < 0.01; **, p < 0.01). B The same transfectants were subjected to cell adhesion, wound healing and invasion. C The control or DSC1-silenced transfectants were subjected to actin polymerization assays. D The same transfectants as in A were subjected to actin polymerization assays. Cell adhesion, migration, invasion or F-actin amount were significantly reduced (◊, p < 0.05; ◊◊, p < 0.01; ◊◊◊, p < 0.001) or enhanced (**, p < 0.01; ***, p < 0.001) after overexpression of CDH17 RAD or DSC1 silencing. Data are representative of three independent experiments