Fig. 4

DSC1 associates with p120-catenin. A Alignment of IA domain of desmogleins and the homologous region of DSC1 and DSC2. Orange letters indicate common or similar residues in desmogleins and desmocollins, red ones in desmogleins and DSC1, and green ones those exclusive of desmogleins. B Confocal microscopy showing the colocalization of DSC1 and p120-catenin in KM12SM and SW620 cells. C The indicated cell lines were transfected with control or DSC1 siRNAs and the extracts were analyzed by Western blot to assess the knock down of DSC1. RhoGDI was used as loading control. DSC1 expression levels were significantly inhibited by the siRNA transfection (*, p < 0.05; **, p < 0.01). D The same transfectants were subjected to immunoprecipitation assays using control or anti-CDH17 antibodies, and the immunoprecipitates analyzed by Western blot to detect p120-catenin, β-catenin and CDH17. The immunoprecipitates were analyzed by Western blot to detect β-catenin and CDH17. B-D Data are representative of three independent experiments