Fig. 1

EGR1 identified as a transcriptional factor target in HCC and EGR1 was downregulated in HCC. A Five candidate transcription factors targets, including EGR1, were identified based on the intersection of differential genes from the TCGA_LIHC dataset, two HCC GEO datasets, and a transcription factor gene set. B The mRNA expression of EGR1 was analyzed in two HCC GEO datasets (GSE36376, GSE84005). C The mRNA expression of EGR1 was examined in 26 pairs of HCC clinical samples. D The protein expression of EGR1 was investigated in 26 pairs of HCC clinical samples, and a scatter plot illustrating the relative quantification of EGR1 protein expression is presented. E The protein expression of EGR1 was confirmed in five pairs of HCC clinical samples using immunohistochemistry staining. F Kaplan–Meier survival analysis of the OS, DSS and RFS rates of patients expressing high or low levels of EGR1 using the Kaplan–Meier plotter survival analysis tool (https://kmplot.com/analysis/). The samples were assigned into EGR1 high/low cohorts by the best available cut-off value. ****P < 0.0001