Fig. 3
From: EGR1 suppresses HCC growth and aerobic glycolysis by transcriptionally downregulating PFKL
EGR1 inhibited HCC cells proliferation in vitro and attenuated tumor growth in vivo. A Western blot assays revealed the presence of EGR1 protein in PLC/PRF5 and HepG2 cells after EGR1 overexpression. B The impact of EGR1 on cell proliferation was assessed using IncuCyte zoom cell proliferation assays. C EDU incorporation assays were performed to evaluate the impact of EGR1 on the growth of HCC cells. D Colony formation experiments were conducted to assess the impact of EGR1 on the ability to form colonies. E HepG2 cells were utilized to investigate the effect of EGR1 on the growth of xenograft tumors. Tumor volume was measured every three days, and after 30 days, the tumors were harvested and weighed. F Ki67 staining of the xenograft tumors was employed to validate the influence of EGR1 overexpression on the growth of xenograft tumors. **P < 0.01, ***P < 0.001, ****P < 0.0001