Fig. 5

EGR1 transcriptionally downregulated PFKL in HCC cells. A The intersection of genes among EGR1 knockout differential genes, EGR1 overexpression differential genes, and glycolysis-related genes was examined. B The mRNA expression of SLC2A3 and PFKL in EGR1 knockout and EGR1 overexpression HCC cells was analyzed using transcriptome sequencing data. C The levels of PFKL mRNA were measured in MHCC97H and PLC/PRF5 cells using RT-qPCR. D The presence of PFKL protein was determined in EGR1 knockout MHCC97H cells and EGR1 overexpressing PLC/PRF5 cells. E A correlation analysis was performed to evaluate the association between EGR1 and PFKL using data from the TCGA database and two GEO datasets. F The GTRD database was used to predict the binding sites of EGR1 in the promoter region of PFKL. G The potential EGR1 binding site in JASPAR is represented by the sequence logo in the top panel, whereas the bottom panel illustrates the mutant PFKL promoter sites. H CHIP assays were conducted in MHCC97H and PLC/PRF5 cells. I Dual luciferase reporter assays provided evidence of the binding of EGR1 in P2 of PFKL. **P < 0.01, ***P < 0.001, ****P < 0.0001, N.S (not significant)