Fig. 4

Immune and genetic profiles of high pSTAT3 expression within TSP^high cases. Box plot showing association between CD45Ro + (A), CD3 + (B), CD8 + (C), FOXP3 + (D), CD66b + (E), CD68 + (F), CD80 + (G), PDL1 + (H), PD1 + (I) and Ki67 + cells (J) and pSTAT3^tyr705 status within TSP^high tumors. Statistical significance was assessed using Mann–Whitney tests and bars represent mean ± standard error of the mean. Corrplot showing the association between pSTAT3 status and clinicopathological characteristics of TSP^high patients (K). Oncoplot showing the top mutated genes between high and low pSTAT3^tyr705 groups within the TSP^high patients (L). Enrichment plots showing the key pathways upregulated in pSTAT3^tyr705 high TSP^high tumors (M–N). Kaplan Meier survival curve showing the association between pSTAT3^tyr705 and TSP score in cohort 3 (p = 0.0019) (O). Volcano plot showing differential gene expression analysis of high versus low pSTAT3^tyr705 within TSP^high cases (P). Box plots showing top upregulated and downregulated genes across pSTAT3^tyr705 high and low groups within TSP^high tumors of cohort 3 (Q). Significance set to p < 0.05*, p < 0.005**, p < 0.0005***, p < 0.00005****