Fig. 1
IGF2BP2 is overexpressed in radioresistant lung cancer cells and promotes radioresistance in lung cancer. A Venn diagrams showing numbers of differentially expressed genes between H460 and H460-RR cells and genes belonging to m6A regulators. B Differential expression analysis of IGF2BP2 between tumor and normal tissues in TCGA lung cancer database. C Scatter plot showing the IHC score of IGF2BP2 protein in LUAD tissue microarray. Normal n = 15, cancer n = 61, unpaired t test. Data are presented as Mean ± SD. D The overall survival of patients with LUAD with different IGF2BP2 levels based on TCGA LUAD database. E H1299 and H1299-RR cells were irradiated at various doses for clonogenic survival assay. SER, sensitization enhancement ratio. n = 3, unpaired t test. Data are presented as Mean ± SD. F RT-qPCR and Western blotting analyses were performed in H1299 (H) and H1299-RR (RR) cells. n = 3, unpaired t test. Data are presented as Mean ± SD. G-K H1299, A549, and H1299-RR cells infected with indicated lentivirus vectors, after puromycin selection, were harvested for Western blotting analysis (G), RT-qPCR analysis (H), neutral comet assay (I and J), and clonogenic survival assay (K). n = 3, one-way ANOVA. Data are presented as Mean ± SD. Scale bar, 50 μm. L H1299-RR cells were transfected with indicated constructs. After puromycin selection, cells were injected subcutaneously into nude mice. The mice were treated with or without IR (10 Gy). Tumor volumes were measured every 3 days. Tumors were harvested, photographed, and weighted at day 21. n = 5, one-way ANOVA. Data are presented as Mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: BP2, IGF2BP2