Fig. 3

SLC7A5 transports Met into cells and increases H3K4me3 enrichment to promote IGF2BP2 expression in lung cancer cells. A H1299 and A549 cells were infected with indicated lentivirus. After puromycin selection, cells were collected for methionine (Met) and S-adenosylmethionine (SAM) assays. n = 3, one-way ANOVA. Data are presented as Mean ± SD. B The ChIP-seq of SETD1A and H3K4me3 on the promoter region of IGF2BP2. C-F H1299 and A549 cells were infected with indicated lentivirus. After puromycin selection, cells were harvested for Western blotting and RT-qPCR analyses. n = 3, one-way ANOVA. Data are presented as Mean ± SD. G and H ChIP-qPCR analyses using SETD1A (G) or H3K4me3 (H) antibodies in A549 cells. n = 3, one-way ANOVA. Data are presented as Mean ± SD. I-L A549 cells were infected with shControl, shSETD1A, shSLC7A5, or treated with complete medium (CM), Met deprivation [Met (-)], or Met restoration [Met ( +)]. Cells were harvested for ChIP-qPCR analyses using H3K4me3 antibody (I and J). And the images for agarose electrophoresis of the products from the corresponding ChIP assay (K and L). n = 3, one-way ANOVA. Data are presented as Mean ± SD. M H1299 and A549 cells were treated with complete medium (CM), Met deprivation [Met (-)], or Met restoration [Met ( +)]. Cells were harvested for Western blotting analysis. ns, not significant, P > 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: SLC, SLC7A5; SET, SETD1A