Fig. 4

The IGF2BP2-SLC7A5 positive feedback loop promotes radioresistance in lung cancer through the AKT/mTOR pathway. A H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were harvested for Western blotting analysis. B-E H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were irradiated at 6 Gy. After 4 h, cells were harvested for neutral comet assay (B and D) and Rad51 foci formation assay (C and E). n = 3, one-way ANOVA. Data are presented as Mean ± SD. Scale bars, 50 μm in D 10 μm in E. F H1299, A549, and H1299-RR cells were infected with indicated lentivirus. After puromycin selection, cells were irradiated at various doses for clonogenic survival assay. n = 3, one-way ANOVA. Data are presented as Mean ± SD. G A549 cells were transfected with indicated constructs. After puromycin selection, cells were injected subcutaneously into nude mice. The mice were treated with or without IR (10 Gy). Tumor volumes were measured every 3 days. Tumors were harvested, photographed, and weighted at day 21. n = 5, one-way ANOVA. Data are presented as Mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: BP2, IGF2BP2; SLC, SLC7A5